EIF2S1 monoclonal antibody (M01), clone 3H12-C11
产品名称： EIF2S1 monoclonal antibody (M01), clone 3H12-C11
英文名称： EIF2S1 monoclonal antibody (M01), clone 3H12-C11
产品编号： H00001965-M01
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Mouse monoclonal antibody raised against a full length recombinant EIF2S1.
- Immunogen:
- EIF2S1 (AAH02513.1, 1 a.a. ~ 315 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
- Sequence:
- MPGLSCRFYQHKFPEVEDVVMVNVRSIAEMGAYVSLLEYNNIEGMILLSELSRRRIRSINKLIRIGRNECVVVIRVDKEKGYIDLSKRRVSPEEAIKCEDKFTKSKTVYSILRHVAEVLEYTKDEQLESLFQRTAWVFDDKYKRPGYGAYDAFKHAVSDPSILDSLDLNEDEREVLINNINRRLTPQAVKIRADIEVACYGYEGIDAVKEALRAGLNCSTENMPIKINLIAPPRYVMTTTTLERTEGLSVLSQAMAVIKEKIEEKRGVFNVQMEPKVVTDTDETELARQMERLERENAEVDGDDDAEEMEAKAED
- Host:
- Mouse
- Reactivity:
- Human
- Isotype:
- IgG1 kappa
- Storage Buffer:
- In 1x PBS, pH 7.2
- Storage Instruction:
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (60.39 KDa) .
- MSDS:
Download
- Publication Reference
- 1.
- IGF2BP1 enhances HCV IRES-mediated translation initiation via the 3'UTR.
Weinlich S, Huttelmaier S, Schierhorn A, Behrens SE, Ostareck-Lederer A, Ostareck DH.RNA. 2009 Aug;15(8):1528-42. Epub 2009 Jun 18.
- Applications
- Western Blot (Cell lysate)
- EIF2S1 monoclonal antibody (M01), clone 3H12-C11 Western Blot analysis of EIF2S1 expression in HeLa ( Cat # L013V1 ).
- Protocol Download
- Western Blot (Cell lysate)
- EIF2S1 monoclonal antibody (M01), clone 3H12-C11. Western Blot analysis of EIF2S1 expression in Hela NE ( Cat # L013V3 ).
- Protocol Download
- Western Blot (Transfected lysate)
- Western Blot analysis of EIF2S1 expression in transfected 293T cell line by EIF2S1 monoclonal antibody (M01), clone 3H12-C11.
Lane 1: EIF2S1 transfected lysate(36.1 KDa).
Lane 2: Non-transfected lysate. - Protocol Download
- Western Blot (Recombinant protein)
- Protocol Download
- Immunofluorescence
enlarge this image- Immunofluorescence of monoclonal antibody to EIF2S1 on HeLa cell. [antibody concentration 10 ug/ml]
- Protocol Download
- Sandwich ELISA (Recombinant protein)
- Detection limit for recombinant GST tagged EIF2S1 is approximately 0.3ng/ml as a capture antibody.
- Protocol Download
- RNAi Knockdown (Antibody validated)
- Western blot analysis of EIF2S1 over-expressed 293 cell line, cotransfected with EIF2S1 Validated Chimera RNAi ( Cat # H00001965-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with EIF2S1 monoclonal antibody (M01), clone 3H12-C11 (Cat # H00001965-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Protocol Download
- Entrez GeneID:
- 1965
- GeneBank Accession#:
- BC002513
- Protein Accession#:
- AAH02513.1
- Gene Name:
- EIF2S1
- Gene Alias:
- EIF-2,EIF-2A,EIF-2alpha,EIF2,EIF2A
- Gene Description:
- eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa
- Omim ID:
- 603907
- Gene Ontology:
- Hyperlink
- Gene Summary:
- The translation initiation factor eIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, eIF2, and GTP. eIF2 is composed of 3 nonidentical subunits, alpha (36 kD), beta (38 kD, MIM 603908), and gamma (52 kD, MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of eIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM
- Other Designations:
- eIF-2-alpha,eukaryotic translation initiation factor 2, subunit 1 (alpha, 35kD )
- Related Disease